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1 25 dihydroxyvitamin d3  (Novus Biologicals)


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    Novus Biologicals 1 25 dihydroxyvitamin d3
    1 25 Dihydroxyvitamin D3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcitriol+elisa+kit/1%2C25-dihydroxyvitamin+D3+(DVD%2FDHVD3)+ELISA+Kit+(Colorimetric)/pmc12752593-322-16-21
    Average 93 stars, based on 5 article reviews
    1 25 dihydroxyvitamin d3 - by Bioz Stars, 2026-11
    93/100 stars

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    Synthesis and characterization of Agg-CLNP formulation. (a) Experimental schematic and chemical structure of aggrecan peptide conjugated calcitriol loaded nanoparticle synthesis. (b) Dynamic light scattering by intensity graph of Agg-CLNP formulation. (c) Dynamic light scattering by intensity plots of Agg-CLNP 7, 14, 21, and 28 days post synthesis. (d) Calcitriol content in Agg-CLNP 0, 7, 14, 21, and 28 days post synthesis as assessed by HPLC. (e) Calcitriol concentration in plasma at indicated time points and in untreated mice. Data in (d) are means ± SD of three technical replicates and in (e) are means ± SD of three experimental replicates. Statistical analyses in (e) were performed using ANOVA with Tukey’s multiple comparison test. Schematic in (a) was composed using BioRender and ChemDraw.

    Journal: ACS Central Science

    Article Title: Immunomodulatory Nanoparticles Enable Combination Therapies To Enhance Disease Prevention and Flare Control in Rheumatoid Arthritis

    doi: 10.1021/acscentsci.5c00723

    Figure Lengend Snippet: Synthesis and characterization of Agg-CLNP formulation. (a) Experimental schematic and chemical structure of aggrecan peptide conjugated calcitriol loaded nanoparticle synthesis. (b) Dynamic light scattering by intensity graph of Agg-CLNP formulation. (c) Dynamic light scattering by intensity plots of Agg-CLNP 7, 14, 21, and 28 days post synthesis. (d) Calcitriol content in Agg-CLNP 0, 7, 14, 21, and 28 days post synthesis as assessed by HPLC. (e) Calcitriol concentration in plasma at indicated time points and in untreated mice. Data in (d) are means ± SD of three technical replicates and in (e) are means ± SD of three experimental replicates. Statistical analyses in (e) were performed using ANOVA with Tukey’s multiple comparison test. Schematic in (a) was composed using BioRender and ChemDraw.

    Article Snippet: Calcitriol ELISA kit (NBP2-82432) was purchased from Novus Biologicals.

    Techniques: Formulation, Concentration Assay, Clinical Proteomics, Comparison

    Agg-CLNP imparts an immunomodulatory phenotype on healthy human MDC in vitro . (a) Experimental schematic of MDC culture. (b and c) Proinflammatory cytokine concentrations in cell culture as measured by (b) IL-6 and (c) TNF. (d–h) Costimulatory molecules (CD40, CD80, and CD86), HLA-2, and CTLA-4 positivity on CD11c + MDC after culture with LPS only, free calcitriol, free dexamethasone, Agg-CLNP and a combination of dexamethasone with Agg-CLNP as measured by (d) CD40, (e) CD80, (f) CD86, (g) HLA-2, and (h) CTLA-4. Data in (b–h) are means ± SD of six technical replicates from representative experiments. Statistical analyses in (b–h) were performed using ANOVA with Tukey’s multiple comparison test. Schematic in (a) was composed using BioRender.

    Journal: ACS Central Science

    Article Title: Immunomodulatory Nanoparticles Enable Combination Therapies To Enhance Disease Prevention and Flare Control in Rheumatoid Arthritis

    doi: 10.1021/acscentsci.5c00723

    Figure Lengend Snippet: Agg-CLNP imparts an immunomodulatory phenotype on healthy human MDC in vitro . (a) Experimental schematic of MDC culture. (b and c) Proinflammatory cytokine concentrations in cell culture as measured by (b) IL-6 and (c) TNF. (d–h) Costimulatory molecules (CD40, CD80, and CD86), HLA-2, and CTLA-4 positivity on CD11c + MDC after culture with LPS only, free calcitriol, free dexamethasone, Agg-CLNP and a combination of dexamethasone with Agg-CLNP as measured by (d) CD40, (e) CD80, (f) CD86, (g) HLA-2, and (h) CTLA-4. Data in (b–h) are means ± SD of six technical replicates from representative experiments. Statistical analyses in (b–h) were performed using ANOVA with Tukey’s multiple comparison test. Schematic in (a) was composed using BioRender.

    Article Snippet: Calcitriol ELISA kit (NBP2-82432) was purchased from Novus Biologicals.

    Techniques: In Vitro, Cell Culture, Comparison

    Synthesis and characterization of Agg-CLNP formulation. (a) Experimental schematic and chemical structure of aggrecan peptide conjugated calcitriol loaded nanoparticle synthesis. (b) Dynamic light scattering by intensity graph of Agg-CLNP formulation. (c) Dynamic light scattering by intensity plots of Agg-CLNP 7, 14, 21, and 28 days post synthesis. (d) Calcitriol content in Agg-CLNP 0, 7, 14, 21, and 28 days post synthesis as assessed by HPLC. (e) Calcitriol concentration in plasma at indicated time points and in untreated mice. Data in (d) are means ± SD of three technical replicates and in (e) are means ± SD of three experimental replicates. Statistical analyses in (e) were performed using ANOVA with Tukey’s multiple comparison test. Schematic in (a) was composed using BioRender and ChemDraw.

    Journal: ACS Central Science

    Article Title: Immunomodulatory Nanoparticles Enable Combination Therapies To Enhance Disease Prevention and Flare Control in Rheumatoid Arthritis

    doi: 10.1021/acscentsci.5c00723

    Figure Lengend Snippet: Synthesis and characterization of Agg-CLNP formulation. (a) Experimental schematic and chemical structure of aggrecan peptide conjugated calcitriol loaded nanoparticle synthesis. (b) Dynamic light scattering by intensity graph of Agg-CLNP formulation. (c) Dynamic light scattering by intensity plots of Agg-CLNP 7, 14, 21, and 28 days post synthesis. (d) Calcitriol content in Agg-CLNP 0, 7, 14, 21, and 28 days post synthesis as assessed by HPLC. (e) Calcitriol concentration in plasma at indicated time points and in untreated mice. Data in (d) are means ± SD of three technical replicates and in (e) are means ± SD of three experimental replicates. Statistical analyses in (e) were performed using ANOVA with Tukey’s multiple comparison test. Schematic in (a) was composed using BioRender and ChemDraw.

    Article Snippet: Plasma samples were analyzed for calcitriol concentration with a calcitriol ELISA detection assay from Novus Biologicals (NBP2–82432) following the manufacturer’s instructions.

    Techniques: Formulation, Concentration Assay, Clinical Proteomics, Comparison

    Agg-CLNP imparts an immunomodulatory phenotype on healthy human MDC in vitro . (a) Experimental schematic of MDC culture. (b and c) Proinflammatory cytokine concentrations in cell culture as measured by (b) IL-6 and (c) TNF. (d–h) Costimulatory molecules (CD40, CD80, and CD86), HLA-2, and CTLA-4 positivity on CD11c + MDC after culture with LPS only, free calcitriol, free dexamethasone, Agg-CLNP and a combination of dexamethasone with Agg-CLNP as measured by (d) CD40, (e) CD80, (f) CD86, (g) HLA-2, and (h) CTLA-4. Data in (b–h) are means ± SD of six technical replicates from representative experiments. Statistical analyses in (b–h) were performed using ANOVA with Tukey’s multiple comparison test. Schematic in (a) was composed using BioRender.

    Journal: ACS Central Science

    Article Title: Immunomodulatory Nanoparticles Enable Combination Therapies To Enhance Disease Prevention and Flare Control in Rheumatoid Arthritis

    doi: 10.1021/acscentsci.5c00723

    Figure Lengend Snippet: Agg-CLNP imparts an immunomodulatory phenotype on healthy human MDC in vitro . (a) Experimental schematic of MDC culture. (b and c) Proinflammatory cytokine concentrations in cell culture as measured by (b) IL-6 and (c) TNF. (d–h) Costimulatory molecules (CD40, CD80, and CD86), HLA-2, and CTLA-4 positivity on CD11c + MDC after culture with LPS only, free calcitriol, free dexamethasone, Agg-CLNP and a combination of dexamethasone with Agg-CLNP as measured by (d) CD40, (e) CD80, (f) CD86, (g) HLA-2, and (h) CTLA-4. Data in (b–h) are means ± SD of six technical replicates from representative experiments. Statistical analyses in (b–h) were performed using ANOVA with Tukey’s multiple comparison test. Schematic in (a) was composed using BioRender.

    Article Snippet: Plasma samples were analyzed for calcitriol concentration with a calcitriol ELISA detection assay from Novus Biologicals (NBP2–82432) following the manufacturer’s instructions.

    Techniques: In Vitro, Cell Culture, Comparison